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Sarkytob — Stem cross-section grape berry
Published: 2011-09-27 18:12:40 +0000 UTC; Views: 2794; Favourites: 35; Downloads: 88
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Description
Please take the time to view it in fullview...Here you can see a micrography of a cross-section through the pedicel of a grape berry (the part connecting the single fruit to the bunch
) at 20-fold magnification. It was stained to contrast the xylem-vessels (the tubes for long-distance water transport in a plant) - the red to pink part of the picture.
It consist of many single images, each showing another sector of the cross-section. It was taken with a motorized micoscop stage which automatically shiftet the slide containing the cross-section to the next section after taking a photo of the current section in view. So it was scanned piecewise.
The original image had 5201x5498 pixel at 200dpi, i reduced both to make it more handsome for the upload
(as uncompressed tif it had roundabout 84 MB).I just like the outcome
(Even so the cross-section wasn't done optimal so it was unequally thick which lead to some out-off-focus parts)
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Comments: 59
Ah, thankyou so much. Yes it is indeed.
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I don't really get what I'm I looking at, but it is fascinating!
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Haha, yes its difficult to interpret if you're not used to these kinds of pictures.
What you see is the cut-surface of a grape berry stem (the connection of the single berry to the bunch) sliced like you would do for timber a tree, or you want to get a disc out of a trunk.
You can see single cells (at least in some parts as the disc was still a bit to thick for optimal light-microscopy)and the red coloured parts are the "tubes" (also cut like a trunk so you only see the "endings") in which the water is transported from the plant to the berry.
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Oh, I get it. I guess? 
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Yeah, as i said its not an optimal section so mostly there are more than one layer of cells viewable.
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Looks indeed somehow abstract, although its completly builded off functional structures.
Thank you very much for the
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What dye did you use?
Looking forward to more fruits of your micrography (pun fully intended
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Phloroglucin and hydrochloric acid, stains a certain molcule of the lignin, which is in this case only present in the xylem vessels.
Probably i don't publish more micrographs as i'm finished my experiments and this was the only interesting one.
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Shame
Wish I could get my hands on a piece of equipment like that!
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Well, yes is a great micoroscope and heavily used in our workgroup, mostly including the fluorescence unit. But the most speciems are prepared to do some sort of measurments on them and not for publishing purposes, so we normally don't mind if parts of the resulting pic is out of focus due to slightly wrinkels and slightly differences in object heigt. And additionally we don't have a microtome or working with embedded objects, meaning we only do handcuts
May i ask you, if you also work in this field somehow?
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To some extent, biotechnology graduate
Now I mostly dabble in college chemistry though
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I see, so i can in future use more specific terms in describing
I'm more into classical plant physiology, but nowadays you nearly can't do anything in the biolocical sciences without some moelcular background or using biotechnolical methods
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Yup I will definitely get that lingo, it's the plant physiology that will need some brushing up ^^'
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Great, this makes things easier
For me its the other way round - our work group is more or less divided into to parts, the classical "paper and pen" one (using balances, photometers etc) and the molecular one. The last time i actuallay worked with molecular methodolgy was for my diploma (5 years or more back in time
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Ha, I just remembered I have a sketchbook full of sketches of plants and their parts under the microscope, from my botany classes.
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Yes, we had to do this too, but my sketching skills are a bit underdeveloped, HaHa, so they really looked awful
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Mine are not that bad I guess. I remember people always taking my sketchbook to copy it, haha. Anyway, I believe there is always room for improvement, so don't let yourself get discouraged. All you need is a bit of practice, just like me.
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Haha, ok, thats a good indicator. It means also that you are a good observer
Thats true - nowadays i haven't to do sketches anymore, hopefully i will finish my graduation till end of the year
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Thank you. I don't consider myself to be an artist, but I do observe well at times.
Best of luck with your graduation. Maybe some day I will graduate too, haha!
Oh and, I see you're a fellow Pratchett fan. Yay!
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Thanks
If you don't start a career before outside the university you will for sure
Oh yes - i could read every single book of him a thousand times, they are so amazing funny and on the same side full of wisdom
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Haha, I doubt that. But thank you for the kind words.
Yes, Terry is a philosopher of a special kind.
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So, now i'm a bit curious - you do a bachelor or master? What are your plans for the time after finishing?
Abolutly agreed.
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I'm a student of agriculture and I remember when I first saw a stem under a microscope... I was amazed how many wonderful colors are in there and how everything looked like art.
I love your photo! Good job!
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Thank you very much
Yeah, i also like this structures. It was actually a picture taken for measurements at the vessels and never meant to be published. As it never will be published in a scientific journal i took the opportunity to upload it here
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It's really brilliant! For some reason it made me really happy. So thank you for uploading it here.
You know, you actually gave me an idea... Next time I manage to get into the lab, I'll try to borrow one slide and turn it into art!
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Great, i'm curious for the outcome
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What type of microscope were you using for the shot? Compound?
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I used our research microscop in the lab - an olympus BX60 (this is the follow up one:[link] ) - which also can be used for fluorescence imaging. Is customized additionally with a motorized stage, so that the object can be moved automatically with defined steps to scan larger objects which don't fit completly in the view window of the objective. As it is used for fluorescence microscopy it is equiped with a camera with a very high aperture.
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0_0 It's beautiful! That's one intense microscope. The only two I've ever used are dissecting and compound. They're both very cool but they don't have anywhere near the same objective or resolution capabilities.
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Yes it is - working with it can be very exiting. But it has its price of course - at least a years salary or more. Alone the lamp for the fluorescence is worth round about 200 Euros and has to be replaced regularly. We use it mainly for analysing cracks in the fruit skin
We have a very well dissecting microscope too - this is even more amazing, you can nearly see the single cells at the highest magnification and that three-dimensional. Unfortunately, the camera can't catch the fantastic 3D view and at the moment i don't have any pics i'm allowed to publish here. Maybe i'll do some especially for DA some day
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Wow, that's a lot of money. I know even some of the cheapest microscopes can cost thousands. Cracks in fruit skin? That's an interesting topic to be studying. That makes sense since I'm sure a lot of the photos being taken are for research purposes. It would be really cool to do something like that. Pictures of cells are rather uncommon on DA and not to mention most people don't ever get to see things like that up close unless they are scientists.
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Yes, thats true - well here is a group called microscopy, which have some pics like this and also fantastic microcraphs taken with an electron microscope.
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0_0 sounds very cool! I'll have to check it out.
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Yes - they have only very few members but some really amazing pics
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0_0 I see what you mean....they do.
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Thank you very much
I'll explain it in german for you and all the others who don't are familar with the english technical terms:
Das ist ein Querschnitt durch das Stielchen einer Weinbeere, fotografiert mit einem Mikroskop bei 20facher Vergrößerung.
Die Details lassen sich besser im Vollbild erkennen (einfach draufklicken
Die dunkleren Zellen in dem äußeren Kreis um den rot gefärbten Zellen gehören zum 2. Leitugnssystem der Pflanze, in dem Zucker und Nährstoffe transportiert werden (und ebenfalls Wasser).
Daran schließt sich nach außen hin Grundgewebe an, in dem unter anderem Stoffwechsel-Vorgänge ablaufen. Ganz außen ist dann das Abschlussgewebe (sozusagen die "Rinde", der Begriff ist in dem Fall aber falsch).
Das Bild ist aus vielen Einzelbildern zusammengesetzt, die automatisch aufgenommen und zusammengesetzt wurden (Der Mikroskoptisch, also da wo der Querschnitt drauflag, wurde Computer gesteuert stückweise unter dem Objektiv verschoben und jeweils ein Bild gemacht, eine Software hat das dann hinterher zusammengesetzt, wenn man genau hinsieht, kann man teilweise die Bildgrenzen der Einzelbilder sehen).
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