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vch41048 — Space in the eyepiece

#cell #cellbiology #fluorescent #microscopy #science
Published: 2017-12-24 21:05:34 +0000 UTC; Views: 211; Favourites: 29; Downloads: 1
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Description Cell: human fibroblasts
Red: microtubules (tubulin cytoskelet)
Green: Golgi apparatus
Blue: nucleus
Microscope: Axiovert 200M (Carl Zeiss)  
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Comments: 9

PapierowySzczur [2018-12-29 20:07:15 +0000 UTC]

From cell culture?

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vch41048 In reply to PapierowySzczur [2018-12-29 22:09:58 +0000 UTC]

Yes. Primary culture. Subcutaneous fibroblasts.

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PapierowySzczur In reply to vch41048 [2018-12-30 16:29:30 +0000 UTC]

It seems different in shape than longer grown ones, and in tissue it's hard to catch the shape. So, I wanted to know. Thank you!

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vch41048 In reply to PapierowySzczur [2018-12-30 21:20:37 +0000 UTC]

It's my pleasure!    There is a possibility that in the primary culture there will be not only fibroblasts, but also other types of cells. But this is easy to check by staining for fibroblast markers. If it is a tissue, not a culture, then you can do the same. Although, it seems to me, it is difficult to confuse fibroblasts with something else. Polarized motion, leading edge, lamella - the main signs. 

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PapierowySzczur In reply to vch41048 [2018-12-30 21:35:42 +0000 UTC]

Thank you again for telling me.

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vch41048 In reply to PapierowySzczur [2019-01-01 14:10:53 +0000 UTC]

 Happy New Year! )))) 

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PapierowySzczur In reply to vch41048 [2019-01-01 17:18:21 +0000 UTC]

Happy New Year!

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xjames7 [2018-01-10 22:31:45 +0000 UTC]

They are like a beautiful drawing! 

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vch41048 In reply to xjames7 [2018-01-11 16:21:09 +0000 UTC]

 

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